Human sIL-6R-SolubleInterleukin 6 Receptor ELISA Kit
Overview of Human sIL-6R-SolubleInterleukin 6 Receptor ELISA Kit
Product name: |
Human sIL-6R-SolubleInterleukin 6 Receptor ELISA Kit |
Reactivity: | Human |
Alternative Names: | IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor |
Assay Type: | Sandwich |
Sensitivity: | 0.83 ng/mL |
Standard: | 120 pg/mL |
Detection Range: | 1.88-120 ng/mL |
Sample Type: | Serum, plasma, tissue homogenates, urine and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Signal transduction;CD & Adhesion molecule;Infection immunity; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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