Human Tie1(Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1) ELISA Kit

Human Tie1(Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1187 Category: Tag:

Overview of Human Tie1(Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1) ELISA Kit

 

Product name: Human Tie1(Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: JTK14; Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1; Tyrosine-protein kinase receptor Tie-1
Assay Type: Sandwich
Sensitivity: 0.62 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Tie1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tie1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human Tie1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tie1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T