Human TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit

Human TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1189 Category: Tag:

Overview of Human TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit

 

Product name: Human TIMP4(Tissue Inhibitors Of Metalloproteinase 4) ELISA Kit
Reactivity: Human
Alternative Names: TIMP Metallopeptidase Inhibitor 4; Tissue Inhibitor of Metalloproteinases 4
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human TIMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T