Human TSH(Thyroid Stimulating Hormone) ELISA Kit

Human TSH(Thyroid Stimulating Hormone) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1163 Category: Tag:

Overview of Human TSH(Thyroid Stimulating Hormone) ELISA Kit

Product name: Human TSH(Thyroid Stimulating Hormone) ELISA Kit
Reactivity: Human
Alternative Names: Thyrotropin
Assay Type: Sandwich
Sensitivity: 0.11 uIU/mL
Standard: 12 uIU/mL
Detection Range: 0.19-12 uIU/mL
Sample Type: Serum
Assay Length: 3.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human TSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSH in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Additional information

Size

48T, 96T, 96T,48T

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