Human TSP-1-Thrombospondin-1 ELISA Kit
Overview of Human TSP-1-hrombospondin-1 ELISA Kit
Product name: |
Human TSP-1-Thrombospondin-1 ELISA Kit |
Reactivity: | Human |
Alternative Names: | TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1 |
Assay Type: | Sandwitch |
Sensitivity: | 53 pg/mL |
Standard: | 8000 ng/mL |
Detection Range: | 125-8000 pg/mL |
Sample Type: | plasma |
Assay Length: | 3.5 h |
Research Area: | Infection immunity;Hematology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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