Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit

Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1256 Category: Tag:

Overview of Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit

Product name: Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
Reactivity: Human
Alternative Names: CD106; INCAM-100; L1CAM
Assay Type: Sandwich
Sensitivity: 0.29 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VCAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human VCAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Read More Description

Additional information

Size

48T, 96T, 96T,48T

Top rated products