Name: Mouse ECF-Eosinophil Chemotactic Factor ELISA Kit - Gencyst
SKU: ELK1010
Availability: InStock

Mouse ECF-Eosinophil Chemotactic Factor ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

Size

Clear

SKU: ELK1010 Category: ELISA Kit Tag: ELISA Kit

Overview of Mouse ECF-Eosinophil Chemotactic Factor ELISA Kit

Product name:	Mouse ECF-Eosinophil Chemotactic Factor ELISA Kit
Reactivity:	Mouse
Alternative Names:	CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Assay Type:	Sandwich
Sensitivity:	3.2 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ECF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ECF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ECF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ECF in the samples is then determined by comparing the OD of the samples to the standard curve.