Name: Mouse EPO-Erythropoietin ELISA Kit - Gencyst
SKU: ELK1012
Availability: InStock

Mouse EPO-Erythropoietin ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

SKU: ELK1012 Category: ELISA Kit Tag: ELISA Kit

Overview of Mouse EPO-Erythropoietin ELISA Kit

Product name:	Mouse EPO-Erythropoietin ELISA Kit
Reactivity:	Mouse
Alternative Names:	EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
Assay Type:	Sandwich
Sensitivity:	2.68 pg/mL
Standard:	400 pg/mL
Detection Range:	6.25-400 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Endocrinology;Hematology;Hormone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EPO in the samples is then determined by comparing the OD of the samples to the standard curve.