Mouse PKM2-Pyruvate Kinase, Muscle ELISA Kit
Mouse PKM2-Pyruvate Kinase, Muscle ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Mouse PKM2-Pyruvate Kinase, Muscle ELISA Kit
| Product name: | Mouse PKM2-Pyruvate Kinase, Muscle ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein |
| Assay Type: | Sandwich |
| Sensitivity: | 0.124 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | Serum, plasma and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Tumor immunity; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PKM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PKM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PKM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PKM2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 48T, 96T |
|---|
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