Name: Rat CD40L-Cluster Of Differentiation 40 Ligand ELISA Kit - Gencyst
SKU: ELK2526
Availability: InStock

Rat CD40L-Cluster Of Differentiation 40 Ligand ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2526 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat CD40L-Cluster Of Differentiation 40 Ligand ELISA Kit

Product name:	Rat CD40L-Cluster Of Differentiation 40 Ligand ELISA Kit
Reactivity:	Rat
Alternative Names:	CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T