Rat CEA(Carcinoembryonic Antigen) ELISA Kit

Rat CEA(Carcinoembryonic Antigen) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1297 Category: Tag:

Overview of Rat CEA(Carcinoembryonic Antigen) ELISA Kit

Product name: Rat CEA(Carcinoembryonic Antigen) ELISA Kit
Reactivity: Rat
Alternative Names: CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
Assay Type: Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5 h
Research Area: Tumor immunity;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CEA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CEA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Rat CEA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CEA in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T