Rat GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

Rat GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1424 Category: Tag:

Overview of Rat GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

Product name: Rat GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit
Reactivity: Rat
Alternative Names: ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor
Assay Type: Sandwich
Sensitivity: 32 pg/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Apoptosis;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDNF in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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