Name: Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit - Gencyst
SKU: ELK1439
Availability: InStock

Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1439 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit

Product name:	Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit
Reactivity:	Rat
Alternative Names:	HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1
Assay Type:	Sandwich
Sensitivity:	6.7 pg/mL
Standard:	1000 ng/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Infection immunity;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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