Rat NAGase-N-Acetyl Beta-D-Glucosaminidase ELISA Kit
Rat NAGase-N-Acetyl Beta-D-Glucosaminidase ELISA Kit
Specification 96T, 48T
Overview of Rat NAGase-N-Acetyl Beta-D-Glucosaminidase ELISA Kit
| Product name: | Rat NAGase-N-Acetyl Beta-D-Glucosaminidase ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | MGEA5; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase |
| Assay Type: | Sandwich |
| Sensitivity: | 0.55 ng/mL |
| Standard: | 100 pg/mL |
| Detection Range: | 1.57-100 ng/mL |
| Sample Type: | urine |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Kidney biomarker; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NAGase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NAGase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NAGase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NAGase in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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