Rat PTH-Parathyroid Hormone ELISA Kit
Rat PTH-Parathyroid Hormone ELISA Kit
Specification 96T, 48T
Overview of Rat I-PTH-IntactParathormone ELISA Kit
| Product name: | Rat I-PTH-IntactParathormone ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone |
| Assay Type: | Sandwich |
| Sensitivity: | 3.93 pg/mL |
| Standard: | 800 pg/mL |
| Detection Range: | 12.5-800 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Metabolic pathway;Endocrinology;Hormone metabolism; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 96T,48T |
|---|