Name: Rat Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit - Gencyst
SKU: ELK1840
Availability: InStock

Rat Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1840 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit

Product name:	Rat Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit
Reactivity:	Rat
Alternative Names:	MADH7; MADH8; Mothers against DPP homolog 8
Assay Type:	Sandwich
Sensitivity:	0.055 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Developmental science;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Smad7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Smad7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Smad7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Smad7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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