Human aFP(Alpha-Fetoprotein) ELISA Kit

Human aFP(Alpha-Fetoprotein) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1301 Category: Tag:

Overview of Human aFP(Alpha-Fetoprotein) ELISA Kit

Product name: Human aFP(Alpha-Fetoprotein) ELISA Kit
Reactivity: Human
Alternative Names: A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
Assay Type: Sandwich
Sensitivity: 0.25 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aFP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human aFP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T