Human AQP5(Aquaporin 5) ELISA Kit

Human AQP5(Aquaporin 5) ELISA Kit

364.00

Specification 96T, 48T

SKU: ELK1471 Category: Tag:

Overview of Human AQP5(Aquaporin 5) ELISA Kit

Product name: Human AQP5(Aquaporin 5) ELISA Kit
Reactivity: Human
Alternative Names: AQP 5; AQP-5; Aquaporin 5; Aquaporin-5
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 pg/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5 h
Research Area: Metabolic pathway;Infection immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AQP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AQP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AQP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AQP5 in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T