Human tPA(Tissue Polypeptide Antigen) ELISA Kit

Human tPA(Tissue Polypeptide Antigen) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1248 Category: Tag:

Overview of Human tPA(Tissue Polypeptide Antigen) ELISA Kit

Product name: Human tPA(Tissue Polypeptide Antigen) ELISA Kit
Reactivity: Human
Alternative Names: PLAT; T-PA; t-plasminogen activator; Alteplase; Reteplase
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human tPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human tPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human tPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human tPA in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T