Mouse bEP-Beta-Endorphin ELISA Kit

Mouse bEP-Beta-Endorphin ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2406 Category: Tag:

Overview of Mouse bEP-Beta-Endorphin ELISA Kit

Product name: Mouse bEP-Beta-Endorphin ELISA Kit
Reactivity: Mouse
Alternative Names: Beta EP; b-EP
Assay Type: Competitive Inhibition
Sensitivity: 4.77 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2 h
Research Area: Signal transduction;Endocrinology;Neuro science;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse bEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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