Overview of Mouse bEP-Beta-Endorphin ELISA Kit
Product name: |
Mouse bEP-Beta-Endorphin ELISA Kit |
Reactivity: |
Mouse |
Alternative Names: |
Beta EP; b-EP |
Assay Type: |
Competitive Inhibition |
Sensitivity: |
4.77 pg/mL |
Standard: |
1000 ng/mL |
Detection Range: |
15.63-1000 pg/mL |
Sample Type: |
Serum, plasma and other biological fluids |
Assay Length: |
2 h |
Research Area: |
Signal transduction;Endocrinology;Neuro science;Hormone metabolism; |
Test Principle: |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse bEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bEP in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix