Mouse PF4(Platelet Factor 4) ELISA Kit

Mouse PF4(Platelet Factor 4) ELISA Kit


Specification 96T, 48T

SKU: ELK1231 Category: Tag:

Overview of Mouse PF4(Platelet Factor 4) ELISA Kit

Product name: Mouse PF4(Platelet Factor 4) ELISA Kit
Reactivity: Mouse
Alternative Names: CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
Assay Type: Sandwich
Sensitivity: 0.068 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, platelet-poor plasma and cell culture supernates
Assay Length: 3.5h
Research Area: Cytokine;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Mouse PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.


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48T, 96T, 96T,48T

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