Rabbit PDGFA-Platelet Derived Growth Factor Subunit A ELISA Kit

Rabbit PDGFA-Platelet Derived Growth Factor Subunit A ELISA Kit

409.00583.00

Specification 96T, 48T

SKU: ELK2439 Category: Tag:

Overview of Rabbit PDGFA-Platelet Derived Growth Factor Subunit A ELISA Kit

Product name: Rabbit PDGFA-Platelet Derived Growth Factor Subunit A ELISA Kit
Reactivity: Rabbit
Alternative Names: PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide
Assay Type: Sandwich
Sensitivity: 10.8 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Cytokine;Tumor immunity;Infection immunity;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PDGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PDGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PDGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PDGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T

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